Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet: Neutralizing mAbs as pre-exposure prophylaxis in k18-hACE2 mice CV1-1, CV1-30, and CV2-75 were assessed to see whether they could confer protection in a mouse model. (A) Experimental timeline. (B) Number of PFUs in the lungs 2 days following challenge. Error bars indicate SD. (C) Viral RNA in lung tissue 2 days after challenge was measured by qPCR and normalized to GAPDH expression. Error bars indicate SD. (D) Kaplan-Meyer survival curve of the viral load/titer in the lungs of remaining mice comparing the various treatment groups. Statistics were determined by one-way ANOVA with Dunnett’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Expressing, Comparison

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet:

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Virus, Infection, Recombinant, Affinity Column, Purification, Staining, Cloning, Cell Isolation, Transfection, Luciferase, Reverse Transcription, Gene Expression, Nested PCR, Sequencing, Expressing, Plasmid Preparation, Software, Microscopy